https://iioablett.pitt.edu/ojs/iioablett/issue/feed 2016-05-09T13:24:04+00:00 ULS e-journals@mail.pitt.edu Open Journal Systems <ul><li><strong><em>IIOAB Letters </em></strong><em>has ceased publication, and this site is no longer accepting submissions.</em></li></ul> https://iioablett.pitt.edu/ojs/iioablett/article/view/19 2016-05-09T13:24:04+00:00 V. P. Zambare vasuzambare@gmail.com S. S. Nilegaonkar ssnilegaonkar@gmail.com P. P. Kanekar kanekarp@rediffmail.com

<p><em>Response surface methodological (RSM) optimization of protease by Pseudomonas aeruginosa MCM B327, increased 1.3-fold activity with 1% inoculum having cell density of 27.57 x 10⁹ cells mL^-1 at pH 7, 30⁰C and 72 h of incubation. Protease enzyme recovered from P. aeruginosa showed characteristic activities against diverse proteins of hide. Enzyme was found to be active with substrates e.g. casein, Bovine serum albumin, gelatin, elastin, haemoglobin but inactive against keratin and collagen. During leather manufacturing, non-collagenase and non-keratinase activities have advantageous in a quality leather and hair saving process, respectively. Increased proteolytic enzyme concentration (0.1-0.5%) in soaking process showed increased water penetration because of hydrolysis of albumin and elastin proteins as indicated by opened fibers in histopathological sections. These findings suggest, protease secreted by P. aeruginosa may have application in soaking operation of leather processing for minimizing harmful deharing chemicals and processing time.</em></p>

2014-01-13T00:00:00+00:00 Copyright (c) https://iioablett.pitt.edu/ojs/iioablett/article/view/20 2016-05-09T13:24:04+00:00 Ashish Polkade apolkade@gmail.com Prafulla Shede prafullashede@gmail.com Pradhnya Kanekar kanekarp@rediffmail.com Prashant Dhakephalkar dprasha@vsnl.com Seema Sarnaik sarnaik@hotmail.com

<p><strong>Abstract</strong></p> <div><p class="AbstractParagraphs">Present study was aimed to design nutrient medium most suitable for isolation and enumeration of microbial flora associated with raw buffalo hide. Skin extract agar (SEA) was designed and standardized on the basis of its chemical analysis. SEA and nutrient medium supplemented with skin extract was inoculated with buffalo hide wash. Total viable count as well as diversity of microbial colonies were enumerated on SEA as well as on nutrient agar and standard plate count agar both supplemented with skin extract (1% v/v). Bacterial strains forming diverse types of colonies on the media tested were identified on the basis of their 16S rRNA gene sequences. The SEA was found to yield higher number of bacteria and to support growth of <em>Acinetobacter, Exiguobacterium </em>and <em>Stenotrophomonas</em> which otherwise difficult to selectively isolate from buffalo hide using nutrient agar and standard plate count agar. Diversity of microbial colonies formed on SEA was significantly higher than that observed on nutrient agar or standard plate count agar. Feasibility of utilizing SEA as a microbiological medium for isolation and identification of microflora from raw buffalo hide was successfully demonstrated. Use of skin extract medium can maximize recovery of taxonomically distinct bacteria from raw buffalo hide. This basic study, with proper manipulations could lead to development of product for enumeration and isolation of bacteria from buffalo hides especially cattle pathogens related to skin diseases.</p></div> <p><strong> </strong></p>

2014-01-13T00:00:00+00:00 Copyright (c)