Protease production and enzymatic soaking of salt-preserved buffalo hides for leather processing

V. P. Zambare, S. S. Nilegaonkar, P. P. Kanekar

Abstract


Response surface methodological (RSM) optimization of protease by Pseudomonas aeruginosa MCM B327, increased 1.3-fold activity with 1% inoculum having cell density of 27.57 x 109 cells mL-1 at pH 7, 300C and 72 h of incubation. Protease enzyme recovered from P. aeruginosa showed characteristic activities against diverse proteins of hide. Enzyme was found to be active with substrates e.g. casein, Bovine serum albumin, gelatin, elastin, haemoglobin but inactive against keratin and collagen. During leather manufacturing, non-collagenase and non-keratinase activities have advantageous in a quality leather and hair saving process, respectively. Increased proteolytic enzyme concentration (0.1-0.5%) in soaking process showed increased water penetration because of hydrolysis of albumin and elastin proteins as indicated by opened fibers in histopathological sections. These findings suggest, protease secreted by P. aeruginosa may have application in soaking operation of leather processing for minimizing harmful deharing chemicals and processing time.


Keywords


protease, enzymatic soaking, leather, Pseudomonas aeruginosa

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References


Adinarayana K, Ellaiah P (2002) Response surface optimization of critical medium components for the production of alkaline protease by a newly isolated Bacillus sp. Journal of Pharma and Pharmaceutical Science 5: 272- 278.

Chauhan B, Gupta R (2004) Application of statistical experimental design for optimization of alkaline protease production from Bacillus sp. RGR-14. Process Biochemistry 39: 2115–2122.

Dutta JR, Dutta PK, Banerjee R (2005). Modeling and optimization of protease production by a newly isolated Pseudomonas sp. using a genetic algorithm Process Biochemistry 40: 879–884.

John DB, Merriline J (2002) Theory and practice of histopathalogical techniques. 5Th Edition. Churchill Publishing Company.

Kamini NR, Hemachander C, Geraldine J, Mala S, Puvanakrishnan R (1999) Microbial enzyme technology as an alternative to conventional chemical in leather industry. Current Science 77: 80-86.not in text

Kanagraj J, Velappan KC, Chandra Babu NK, Sadulla S (2006) Solid waste generation in the leather industry and its utilization for cleaner environment- a review. Journal of Scientific and Industrial Research 65: 541- 548.

Luthra YK (2006) Indian leather industry. Leather Age March: 69-71.

Muthian PL, Ramanathan N, Nayudamma Y (1968) Biochemical studies of the skin samples obtained from different sites on various animals. Journal of American Leather Chemists Association 63: 38-47.

Nielsen PH (2006) Environmental assessment of enzyme application in the tanning industry. Leather International August- September: 18-24.

Puri S, Beg QK, Gupta R (2002) Optimization of alkaline protease production from Bacillus sp. by response surface methodology. Current Microbiology 44: 286–290.

Puvankrishnan R and Dhar SC (1988). Enzymes in soaking, dehairing, bating and degreasing. In: Enzyme Technology in Beamhouse Practice. Central Leather Research Institute, Madras.

Rao YK, Lu SC, Liu BL and Tzeng YM (2006). Enhanced production of an extracellular proteasefrom Beauveria bassiana by optimization of cultivation processes. Biochemical Engineering Journal 28: 57–66.

Reddy LVA, Wee Y-J, Yun J-S, Ryu H-W (2008) Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 though Plackett-Burman and response surface methodological approaches. Bioresource Technology 99: 2242-2249.

Sachar LA, Winter KK, Sicher N, Frankel S (1995) Photometric method for estimation of elastase activity. Proceeding of Society of Experimental Medical Biology 90: 323-326.

Sarath G, Motte RSDL, Wagner FW (1996) Hemoglobin hydrolysis assay. In: Proteolytic Enzymes- A Practical Approach, Beynon RJ, Bond JS (Eds). Oxford IRL Press, New York.

Sharphouse JH (1983) Leather Technician’s Handbook. 2nd Edn. Leather Producers Association, London.

Takami H, Akiba T, Horikoshi K (1990) Characterization of an alkaline protease from Bacillus sp. No. AH- 101. Applied Microbiology and Biotechnology 33: 519- 523.

Thanikaivelan P, Rao JR, Nair BU and Ramasami T (2003). Approach towards zero discharge tanning: role of concentration on the development of eco-friendly liming- reliming process. Journal of Cleaner Production 11:79- 90.

Saxena R, Singh R (2010) Statistical optimization of conditions for protease production from Bacillus sp. Acta Biologica Szegediensis 54 (2): 135-141.

Woessner JF (1961) The determination of hydroxyproline in tissue and protein samples containing small portions of this iminoacids. Archives in Biochemistry and Biophysics 93: 440- 447.

Zambare VP, Nilegaonkar SS, Kanekar PP (2011) Production optimization and purification of a novel extracellular protease from Pseudomonas aeruginosa MCM B-327. New Biotechnology 28 (2): 173-181

Zambare VP, Nilegaonkar SS, Kanekar PP (2011a) Use of agroresidues for protease production and application in degelatinazation. Research Journal of BioTechnology 6(2): 62-65




DOI: https://doi.org/10.5195/iioablett.2013.19

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