Effect of Various Nutritional Supplements on Hyaluronic Acid Production

Authors

  • V J Aroskar Department of Microbiology, Mithibai College of Arts, Chauhan Institute of Science & Amruthben Jivanlal College of Commerce and Economics,Vile Parle (W), Mumbai 400 056
  • S D Kamat Head,Department of Microbiology, Mithibai College of Arts, Chauhan Institute of Science & Amruthben Jivanlal College of Commerce and Economics,Vile Parle (W), Mumbai 400 056
  • D V Kamat Department of Microbiology, Mithibai College of Arts, Chauhan Institute of Science & Amruthben Jivanlal College of Commerce and Economics,Vile Parle (W), Mumbai 400 056

DOI:

https://doi.org/10.5195/iioablett.2012.18

Keywords:

Streptococcus zooepidemicus, Hyaluronic acid, L-arginine HCl, L-glutamine, Sodium glucuronate, Shake flask fermentation

Abstract

Effect of various nutritional supplementations on production of hyaluronic acid (HA) using Streptococcus equi subsp. zooepidemicus ATCC 39920 was evaluated at shake flask. Among the different carbon sources used, maltodextin showed HA of 0.9 g/L followed by 0.7 g/L for dextrose. L-arginine HCl at 2.0 g/L increased the yield of HA from 0.7 g/L to 0.85 g/L. L-arginine and L-glutamine showed the yield of HA of 0.7 g/L and 1.0 g/L, respectively at 2.0 g/L concentration. L-glutamine (2.0 g/L) and uridine (0.75 g/L) showed HA yield of 0.8 and 1.045 g/L, respectively. Addition of sodium glucuronate at 0.25 g/L concentration at 8 h, the yield of HA was increased from 0.7 g/L to 0.85 g/L. Pantothenic acid at concentration of 8.33 mg/L supplemented to the P10 medium, yield of 0.87 g/L of HA was obtained. Active dry yeast at 5.0 g/L increased yield of HA from 0.7 g/L to 0.85 g/L. Tween 80 at higher concentration reduced HA production. The addition of MgSO4.7H2O at 1.0 g/L improved the production of HA. Other metal ions like manganese, ferrous, copper, and zinc have no significant effect on HA production. Thus, it has been found that various nutritional supplements have effect on production of HA using S. equi subsp. zooepidemicus ATCC 39920

 

Author Biographies

V J Aroskar, Department of Microbiology, Mithibai College of Arts, Chauhan Institute of Science & Amruthben Jivanlal College of Commerce and Economics,Vile Parle (W), Mumbai 400 056

Department of Microbiology

S D Kamat, Head,Department of Microbiology, Mithibai College of Arts, Chauhan Institute of Science & Amruthben Jivanlal College of Commerce and Economics,Vile Parle (W), Mumbai 400 056

Department of Microbiology,

D V Kamat, Department of Microbiology, Mithibai College of Arts, Chauhan Institute of Science & Amruthben Jivanlal College of Commerce and Economics,Vile Parle (W), Mumbai 400 056

Department of Microbiology,

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Published

2013-01-16

Issue

Vol. 2 No. 1 (2012)

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Articles

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